Palaeoecological records are required to test ecological hypotheses necessary for conservation strategies as short-term observations can insufficiently capture natural variability and identify drivers of biotic change. Here, we demonstrate the importance of an integrated conservation palaeobiology approach when making validated decisions for conservation and mitigating action. Our model system is the Razim-Sinoie lake complex (RSL) in the Danube Delta (Black Sea coast, Romania), a dynamic coastal lake system hosting unique Pontocaspian mollusc species that are now severely under threat. The Pontocaspians refer to an endemic species group that evolved in the Black Sea and Caspian Sea basins under reduced salinity settings over the past few million years. The natural, pre-industrial RSL contained a salinity gradient from fresh to mesohaline (18ppm) until human intervention reduced the inflow of mesohaline Black Sea water into the lake system. We reconstruct the evolution of the RSL over the past 2000 years from integrated sedimentary facies and faunal analyses based on 11 age-dated sediment cores and investigate the response of mollusc species and communities to those past environmental changes. Three species associations ("marine", "Pontocaspian" and "freshwater") exist and their spatio-temporal shifts through the system are documented. Variable salinity gradients developed, with marine settings (and faunas) dominating in the southern part of the system and freshwater conditions (and faunas) in the northern and western parts. Pontocaspian species have mostly occurred in the centre of the RSL within the marine-freshwater salinity gradient. Today, freshwater species dominate the entire system, and only a single Pontocaspian species (Monodacna colorata) is found alive. We show that the human-induced reduced marine influence in the system has been a major driver of the decline of the endemic Pontocaspian biota. It urges improved conservation action by re-establishing a salinity gradient in the lake system to preserve these unique species.

The Caspian Sea, the largest isolated lake in the world, witnessed drastic lake-level variations during the Quaternary. This restricted basin appears very sensitive to lake-level variations, due to important variations in regional evaporation, precipitation and runoff. The amplitude, frequency and drivers of these lake-level changes are still poorly documented and understood. Studying geological records of the Caspian Sea might be the key to better comprehend the complexity of these oscillations. The Hajigabul section documents sediment deposited on the northern margin of the Kura Basin, a former embayment of the Caspian Sea. The 2035 m thick, well-exposed section was previously dated by magneto-biostratigraphic techniques and provides an excellent record of Early Pleistocene environmental, lake-level and climate changes. Within this succession, the 1050 m thick Apsheronian regional stage, between ca 2·1 Ma and 0·85 Ma, represents a particular time interval with 20 regressive sequences documented by sedimentary and palaeontological changes. Sequences are regressing from offshore to coastal, lagoonal or terrestrial settings and are bounded by abrupt flooding events. Sediment reveals a low energy, wave-dominated, reflective beach system. Wave baselines delimiting each facies association appear to be located at shallower bathymetries compared to the open ocean. Water depth estimations of the wave baselines allow reconstruction of a lake-level curve, recording oscillations of ca 40 m amplitude. Cyclostratigraphic analyses display lake-level frequency close to 41 kyr, pointing to allogenic forcing, dominated by obliquity cycles and suggesting a direct or indirect link with high-latitude climates and environments. This study provides a detailed lake-level curve for the Early Pleistocene Caspian Sea and constitutes a first step towards a better comprehension of the magnitude, occurrence and forcing mechanisms of Caspian Sea lake-level changes. Facies models developed in this study regarding sedimentary architectures of palaeocoastlines affected by repeated lake-level fluctuations may form good analogues for other (semi-)isolated basins worldwide.

Sedimentological facies models for (semi-)isolated basins are less well developed than those for marine environments, but are critical for our understanding of both present-day and ancient deltaic sediment records in restricted depositional environments. This study considers an 835 m thick sedimentary succession of mid-Pliocene age, which accumulated in the Dacian Basin, a former embayment of the Black Sea. Detailed sedimentological and palaeontological analyses reveal a regression from distal prodelta deposits with brackish water faunas to delta-top deposits with freshwater faunas. Sediments contain frequent hyperpycnal plumes and an enrichment in terrestrial organic material, ichnofossils and in situ brackish and freshwater faunas. Deltaic progradation created thin, sharply-based sand bodies formed by multiple terminal distributary channels, covering a wide depositional area. The system experienced frequent delta-lobe switching, resulting in numerous thin parasequences. Parasequences are overlain by erosive reddish oxidized sand beds, enriched in broken, abraded brackish and freshwater shells. These beds were formed after sediment starvation, on top of abandoned delta lobes during each flooding event. A robust magnetostratigraphic time frame allowed for comparison between the observed sedimentary cyclicity and the amplitude and frequency of astronomical forcing cycles. Our results indicate that parasequence frequencies are significantly higher than the number of time equivalent astronomical cycles. This suggests that delta-lobe switching was due to autogenic processes. We consider the observed facies architecture typical for a delta prograding on a low-gradient slope into a shallow, brackish, protected, semi-isolated basin. Furthermore, in the absence of significant wave and tidal influence, sediment progradation in such a protected depositional setting shaped a delta, strongly river-dominated.