Biochemical oxidation and reduction are the principle of biological water and wastewater treatment, in which electron donor and/or acceptor shall be provided. Elemental sulfur (S0) as a non-toxic and easily available material with low price, possesses both reductive and oxidative characteristics, suggesting that it is a suitable material for water and wastewater treatment. Recent advanced understanding of S0-respiring microorganisms and their metabolism further stimulated the development of S0-based technologies. As such, S0-based biotechnologies have emerged as cost-effective and attractive alternatives to conventional biological methods for water and wastewater treatment. For instance, S0-driven autotrophic denitrification substantially lower the operational cost for nitrogen removal from water and wastewater, compared to the conventional process with exogenous carbon source supplementation. The introduction of S0 can also avoid secondary pollution commonly caused by overdose of organic carbon. S0 reduction processes cost-effectively mineralize organic matter with low sludge production. Biological sulfide production using S0 as electron acceptor is also an attractive technology for metal-laden wastewater treatment, e.g. acid mine drainage. This paper outlines an overview of the fundamentals, characteristics and advances of the S0-based biotechnologies and highlights the functional S0-related microorganisms. In particular, the mechanisms of microorganisms accessing insoluble S0 and feasibility to improve S0 bio-utilization efficiency are critically discussed. Additionally, the research knowledge gaps, current process limitations, and required further developments are identified and discussed.@en

Global water security is a severe issue that threatens human health and well-being. Finding sustainable alternative water resources has become a matter of great urgency. For coastal urban areas, desalinated seawater could serve as a freshwater supply. However, since 20%–30% of the water supply is used for flushing waste from the city, seawater with simple treatment could also partly replace the use of freshwater. In this work, the freshwater saving potential and environmental impacts of the urban water system (water-wastewater closed loop) adopting seawater desalination, seawater for toilet flushing (SWTF), or reclaimed water for toilet flushing (RWTF) are compared with those of a conventional freshwater system, through a life-cycle assessment and sensitivity analysis. The potential applications of these processes are also assessed. The results support the environmental sustainability of the SWTF approach, but its potential application depends on the coastal distance and effective population density of a city. Developed coastal cities with an effective population density exceeding 3000 persons·km−2 and located less than 30 km from the seashore (for the main pipe supplying seawater to the city) would benefit from applying SWTF, regardless of other impact parameters. By further applying the sulfate reduction, autotrophic denitrification, and nitrification integrated (SANI) process for wastewater treatment, the maximum distance from the seashore can be extended to 60 km. Considering that most modern urbanized cities fulfill these criteria, the next generation of water supply systems could consist of a freshwater supply coupled with a seawater supply for sustainable urban development.@en