Regulating the rational wettability on gas-diffusion electrodes (GDEs) plays a pivotal role to improve the efficiency of CO₂RR via fine-tuning the reaction zone and boosting the formation of triple-phase interfaces. Herein, we present a wettability regulation strategy that modulates the triple-phase reaction zone in the catalyst layer of GDEs. This strategy was employed on a flow-through hollow fiber GDE coated with a Bi-embedded catalyst layer. Compared to other ex-situ methods (e.g., adding wetting agents) affecting the bulk of electrocatalysts or catalyst layer, we create distinctive hydrophilic-hydrophobic regions within the catalyst layer. Catalyst layer with hydrophilic-hydrophobic regions outperforms the fully hydrophilic one by facilitating the species transport, boosting triple-phase interface formation, and maximizing the active sites. This regulation strategy showed stable wettability during CO₂RR cathodic conditions, evidenced by the direct measurement of penetration depth. The electrode with the regulated wettability exhibited over 80% catalyst utilization and 4 times higher formate partial current density (~150 mA cm⁻² with FE_formate> 90%) compared to the untreated electrode, outperforming other GDEs employed for CO₂RR to formate in the same concentrations of bicarbonate. The finding of this versatile microenvironment regulation strategy can be extended to GDEs used for other gas-phase reactions.