IL-21/IL-21R signaling is crucial in various immune diseases and cellular development, however, its role in bacterial pneumonia remains unclear. Here, IL-21R knockout (IL-21R^−/−) mice were more susceptible to Actinobacillus pleuropneumoniae (APP) than wild-type (WT) mice. High-dimensional mass cytometry analysis revealed that IL-21R deficiency inhibited neutrophil activation, decreased the numbers of monocytes and proinflammatory macrophages, and augmented the defective CD3^low T cells in the lungs. Intracellular cytokine staining showed decreased IFN-γ/TNF-α/IL-6 production in IL-21R^−/− mice, particularly in CD8⁺ T cells. Furthermore, a previously unrecognized Ly6C⁺Ly6G⁺CD4⁺ T cell subset emerged only in the lungs of WT mice post-APP infection, which was in an activated status with stronger secretion capacities of IL-10, IL-21, granzyme B, and perforin by flow cytometry. These cells polarized macrophages into M2- or M1- phenotype without/with infection, respectively, and enhanced proliferation, phagocytosis, and macrophage extracellular traps/ROS-mediated bactericidal activity of macrophages against-APP, Klebsiella pneumoniae, or Escherichia coli infection. Thus, our study demonstrated that IL-21 drives the differentiation of neutrophils, monocytes, and macrophages into pro-inflammatory subsets. IL-21-induced Ly6C⁺Ly6G⁺CD4⁺ T cells cooperate with macrophages to enhance bacterial clearance, providing a promising target for preventing bacterial pneumonia. (Figure presented.)

Porcine contagious pleuropneumonia caused by Actinobacillus pleuropneumoniae (APP) is a fatal respiratory disease that threatens the worldwide farming industry’s health. The immune responses of extrapulmonary tissues play an important role in developing porcine contagious pleuropneumonia; however, the immune responses of extrapulmonary tissues induced by APP are rarely uncovered. Here, we used high-dimensional mass cytometry to investigate the immune cell response in the spleen and peripheral blood during APP infection in mice. We found that the immune response triggered by APP was highly tissue-specific. Numerous infection time- or tissue-specific immune cell clusters, including previously unrecognized ones, were also identified in the spleen and peripheral blood. Integrative analysis of splenic lymphoid and myeloid cell clusters maps the dynamic immune response cellular network during APP infection. Surprisingly, during the early stages of APP infection, the majority of the top 6 cell clusters contributing to the infection time-specificity in the spleen were adaptive immune cell clusters rather than innate immune cell clusters, among which CD24hiMHCII+CD8+TEM cells exhibited a stronger expression of IFN-γ, IL-17A, and IL-10 compared to the CD24lo compartment. In peripheral blood, there was unprecedented heterogeneity in the immune cell composition. Also, peripheral immune cell clusters closely related to the severity of APP infection were identified. In summary, our data provide a systemic and comprehensive overview of the immune responses to APP infection in the spleen and peripheral blood. This provides a foundation for understanding the immune pathogenesis of APP and identifying potential diagnostic biomarkers and therapeutic targets.

Meningitis caused by Streptococcus suis serotype 2 (SS2) in humans and pigs is an acute nervous disorder associated with serious sequelae. Bacterial meningitis is tightly associated with immune cell responses and the local immune microenvironment. However, the dynamic changes of the immune system during the disease progression in the brain remains unclear. Here, single-cell mass cytometry analyses are used to comprehensively profile the composition and phenotypes of female mouse brain immune cells at different stages of SS2 meningitis. Ten major immune cell lineages are identified among which T cells and dendritic cells significantly increased during meningitis, with B cells increasing in the late stage. Specifically, SS2⁺PD-L1⁺ neutrophils with strong phagocytosis, bactericidal and apoptotic effects accumulate in the acute phase of SS2 infection. Microglia sequentially display the features of homeostasis, proliferation, and activation (enhanced MHCII and TLR2 signals and TNF-α secretion) during the process of meningitis. Both border-associated and monocyte-derived macrophages contribute to the process of SS2-induced meningitis, exhibiting upregulation of CD38 and MHCII. Interestingly, CD11c⁺CD8⁺T cells are the main contributor of IFN-γ and specifically appeared during SS2 infection. In addition, the appearance of other lymphocytes such as CCR6^+/lo B cells, CX3CR1⁺ NK and MHCII⁺ ILC3 are related to the progression of meningitis. Moreover, correlation analysis between the composition of immune cell clusters and the SS2 infection process yield a dynamic immune landscape in which key immune clusters, including some previously unidentified, mark different stages of infection. Together, these data reveal the unique infection-stage immune microenvironment during the progression of meningitis caused by SS2 and provide resources for the analysis of immunological pathogenesis, potential diagnostic markers and therapeutic targets for bacterial meningitis. (Figure presented.)