This paper investigates the effect of temperature and hybridization on the impact damage evolution and post-impact residual strength of hemp/epoxy, basalt/epoxy and their hybrid laminates, using mechanical and acoustic emission (AE) based analysis. To start with, the specimens were impacted by a drop weight impact tower machine at two temperatures of 30 °C and 65 °C and then they were subjected to a three-point bending test for the assessment of their residual strength, while online AE signals were recorded during the test. The mechanical behavior of the laminates was evaluated through measurement of the impact force and absorbed energy. AE response of the slope of cumulative rise angle (RA) was used for identification of the severity of the impact-induced damage in the laminates. In addition, the sentry function was computed on the basis of the correlation between the mechanical strain energy stored in the materials and the acoustic energy propagates by fracture events, enabled evaluation of the amount of impact-induced damage. These results showed the hybridized laminates having a better resistance to impact damage at the elevated temperature (65 °C) compared with the non-hybridized laminates, whereas, in the case of the ambient temperature (30 °C), basalt/epoxy laminates had a higher impact damage resistance than other configurations. This study reveals the capability of the proposed AE-based methods to investigate the effect of temperature and hybridization of composite laminates.