Qiao, Li (author), Luo, Zhiyuan (author), Wang, Ru (author), Pei, Xiaolin (author), Wu, Shujiao (author), Chen, Haomin (author), Xie, Tian (author), Sheldon, R.A. (author), Wang, Anming (author) Two non-canonical amino acids (ncAAs) with bio-orthogonal reactive groups, namely, p-azido-l-phenylalanine (p-AzF) and p-propargyloxy-l-phenylalanine (p-PaF), were genetically inserted into an aldo-keto reductase (AKR) and an alcohol dehydrogenase (ADH), respectively, at two preselected sites for each enzyme. The variants were expressed in...
journal article 2023