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NINscope, a versatile miniscope for multi-region circuit investigations

Journal Article (2020)
Author(s)

Andres de Groot (Netherlands Institute for Neuroscience)

Bastijn Jg van den Boom (Netherlands Institute for Neuroscience, Universiteit van Amsterdam)

Romano M. van Genderen (Student TU Delft)

Joris Coppens (Netherlands Institute for Neuroscience)

John van Veldhuijzen (Netherlands Institute for Neuroscience)

Joop Bos (Netherlands Institute for Neuroscience)

Hugo Hoedemaker (Netherlands Institute for Neuroscience)

Mario Negrello (TU Delft - Education and Student Affairs, Erasmus MC)

Ingo Willuhn (Netherlands Institute for Neuroscience, Universiteit van Amsterdam)

Chris I. De Zeeuw (Netherlands Institute for Neuroscience, Erasmus MC)

Tycho M. Hoogland (Netherlands Institute for Neuroscience, Erasmus MC)

DOI related publication
https://doi.org/10.7554/eLife.49987 Final published version
More Info
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Publication Year
2020
Language
English
Volume number
9
Article number
e49987
Downloads counter
300
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Institutional Repository
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Abstract

Miniaturized fluorescence microscopes (miniscopes) have been instrumental to monitor neural signals during unrestrained behavior and their open-source versions have made them affordable. Often, the footprint and weight of open-source miniscopes is sacrificed for added functionality. Here, we present NINscope: a light-weight miniscope with a small footprint that integrates a high-sensitivity image sensor, an inertial measurement unit and an LED driver for an external optogenetic probe. We use it to perform the first concurrent cellular resolution recordings from cerebellum and cerebral cortex in unrestrained mice, demonstrate its optogenetic stimulation capabilities to examine cerebello-cerebral or cortico-striatal connectivity, and replicate findings of action encoding in dorsal striatum. In combination with cross-platform acquisition and control software, our miniscope is a versatile addition to the expanding tool chest of open-source miniscopes that will increase access to multi-region circuit investigations during unrestrained behavior.