Nanopore Sequencing of Peptides
Terminal Conjugation and PTM Detection
J. Ritmejeris (TU Delft - BN/Cees Dekker Lab)
C. Dekker – Promotor (TU Delft - BN/Cees Dekker Lab)
C. Joo – Promotor (TU Delft - BN/Chirlmin Joo Lab)
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Abstract
In this thesis, I present the development of biophysical and chemical strategies to enable single-molecule protein sequencing using biological nanopores. While genomic sequencing has transformed biology by enabling the massive-scale amplification and sequencing of nucleic acids, a comparable revolution in proteomics has remained elusive. Proteins cannot be amplified, and existing techniques – most prominently mass spectrometry – rely on ensemble averaging, destroying labile modifications and masking molecular heterogeneity. The aim of this thesis was to develop a framework for direct amplification-free analysis of individual peptides using nanopore-based sequencing....
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File under embargo until 15-04-2027