Cytochrome cbb<sub>3 </sub>of Thioalkalivibrio is a Na<sup>+</sup>-pumping cytochrome oxidase

Journal article (2015)

Authors

Maria S. Muntyan Moscow State University

Dmitry A. Cherepanov Moscow State University

M. Malinen University of Turku

Dmitry A. Bloch University of Helsinki, Moscow State University

Dimitry Y. Sorokin BT/Environmental Biotechnology - , Russian Academy of Sciences

Inna I. Severina Moscow State University

Tatiana V. Ivashina Russian Academy of Sciences

Reijo Lahti University of Turku

Gerard Muyzer Universiteit van Amsterdam

Vladimir P. Skulachev Moscow State University

Research Group

BT/Environmental Biotechnology () (TU Delft)

Alkaliphily Cbb<inf>3</inf>-type oxidase Cytochrome c oxidase Sodium pumping

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Published Date

23-06-2015

Language

English

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Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Environmental Biotechnology

Abstract

Cytochrome c oxidases (Coxs) are the basic energy transducers in the respiratory chain of the majority of aerobic organisms. Coxs studied to date are redox-driven proton-pumping enzymes belonging to one of three subfamilies: A-, B-, and C-type oxidases. The C-type oxidases (cbb3 cytochromes), which are widespread among pathogenic bacteria, are the least understood. In particular, the proton-pumping machinery of these Coxs has not yet been elucidated despite the availability of X-ray structure information. Here, we report the discovery of the first (to our knowledge) sodium-pumping Cox (Scox), a cbb3 cytochrome from the extremely alkaliphilic bacterium Thioalkalivibrio versutus. This finding offers clues to the previously unknown structure of the ionpumping channel in the C-type Coxs and provides insight into the functional properties of this enzyme.