Cytochrome cbb3 of Thioalkalivibrio is a Na+-pumping cytochrome oxidase
Maria S. Muntyan (Moscow State University)
Dmitry A. Cherepanov (Moscow State University)
Anssi M. Malinen (University of Turku)
Dmitry A. Bloch (University of Helsinki, Moscow State University)
D. Sorokin (TU Delft - Applied Sciences, Russian Academy of Sciences)
Inna I. Severina (Moscow State University)
Tatiana V. Ivashina (Russian Academy of Sciences)
Reijo Lahti (University of Turku)
Gerard Muyzer (Universiteit van Amsterdam)
Vladimir P. Skulachev (Moscow State University)
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Abstract
Cytochrome c oxidases (Coxs) are the basic energy transducers in the respiratory chain of the majority of aerobic organisms. Coxs studied to date are redox-driven proton-pumping enzymes belonging to one of three subfamilies: A-, B-, and C-type oxidases. The C-type oxidases (cbb3 cytochromes), which are widespread among pathogenic bacteria, are the least understood. In particular, the proton-pumping machinery of these Coxs has not yet been elucidated despite the availability of X-ray structure information. Here, we report the discovery of the first (to our knowledge) sodium-pumping Cox (Scox), a cbb3 cytochrome from the extremely alkaliphilic bacterium Thioalkalivibrio versutus. This finding offers clues to the previously unknown structure of the ionpumping channel in the C-type Coxs and provides insight into the functional properties of this enzyme.