Light-sheet optimization for microscopy
Dean Wilding (TU Delft - Team Raf Van de Plas)
Paolo Pozzi (TU Delft - Team Raf Van de Plas)
Oleg Soloviev (Flexible Optical B.V., TU Delft - Team Raf Van de Plas)
Gleb Vdovine (Flexible Optical B.V., TU Delft - Team Raf Van de Plas)
Michel Verhaegen (TU Delft - Team Raf Van de Plas)
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Abstract
Aberrations, scattering and absorption degrade the performance light-sheet fluorescence microscopes (LSFM). An adaptive optics system to correct for these artefacts and to optimize the light-sheet illumination is presented. This system allows a higher axial resolution to be recovered over the field-of-view of the detection objective. It is standard selective plane illumination microscope (SPIM) configuration modified with the addition of a spatial light modulator (SLM) and a third objective for the detection of transmitted light. Optimization protocols use this transmission light allowing the extension the depth-of-field and correction of aberrations whilst retaining a thin optical section.