Light-sheet optimization for microscopy
Dean Wilding (TU Delft - Mechanical Engineering)
Paolo Pozzi (TU Delft - Mechanical Engineering)
Oleg Soloviev (Flexible Optical B.V., TU Delft - Mechanical Engineering)
Gleb Vdovine (Flexible Optical B.V., TU Delft - Mechanical Engineering)
Michel Verhaegen (TU Delft - Mechanical Engineering)
More Info
expand_more
Other than for strictly personal use, it is not permitted to download, forward or distribute the text or part of it, without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license such as Creative Commons.
Abstract
Aberrations, scattering and absorption degrade the performance light-sheet fluorescence microscopes (LSFM). An adaptive optics system to correct for these artefacts and to optimize the light-sheet illumination is presented. This system allows a higher axial resolution to be recovered over the field-of-view of the detection objective. It is standard selective plane illumination microscope (SPIM) configuration modified with the addition of a spatial light modulator (SLM) and a third objective for the detection of transmitted light. Optimization protocols use this transmission light allowing the extension the depth-of-field and correction of aberrations whilst retaining a thin optical section.