Towards the development of a droplet micro-reactor for industrial relevant screening in biotechnology

Conference paper (2019)

Authors

K. Totlani ChemE/Product and Process Engineering - AS
W.M. van Gulik BT/Industriele Microbiologie - AS
M.T. Kreutzer ChemE/Chemical Engineering
V. van Steijn ChemE/Product and Process Engineering - AS
Research Group
ChemE/Product and Process Engineering (AS) (TU Delft)
Microfluidics Coalescence Screening Cell growth Microdroplets Nutrient feeding
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Published Date

2019

Language

English

Reuse Rights

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Faculty

Applied Sciences

Department

ChemE/Chemical Engineering

Research Group

ChemE/Product and Process Engineering

Abstract

We report the development of a droplet-based microfluidic device, which enables long term culturing of microorganisms inside water in oil microdroplets under semi-continuous conditions. Firstly, a microdroplet containing yeast cells is trapped on chip. After an initial incubation phase, this cell laden droplet is coalesced with smaller nutrient droplets to enable cultivation and time-lapsing under semi-continuous conditions. We demonstrate a proof-of-principle study on a sequential batch experiment, which lays a sound foundation for screening of microorganisms under semi-continuous conditions.