Detection of phosphorylation post-translational modifications along single peptides with nanopores
Ian C. Nova (TU Delft - Applied Sciences, Kavli institute of nanoscience Delft)
Justas Ritmejeris (TU Delft - Applied Sciences, Kavli institute of nanoscience Delft)
Henry Brinkerhoff (University of Washington, Kavli institute of nanoscience Delft, TU Delft - Applied Sciences)
Theo J.R. Koenig (TU Delft - Applied Sciences, Kavli institute of nanoscience Delft, TU Delft - Applied Sciences)
Jens H. Gundlach (University of Washington)
Cees Dekker (Kavli institute of nanoscience Delft, TU Delft - Applied Sciences)
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Abstract
Current methods to detect post-translational modifications of proteins, such as phosphate groups, cannot measure single molecules or differentiate between closely spaced phosphorylation sites. We detect post-translational modifications at the single-molecule level on immunopeptide sequences with cancer-associated phosphate variants by controllably drawing the peptide through the sensing region of a nanopore. We discriminate peptide sequences with one or two closely spaced phosphates with 95% accuracy for individual reads of single molecules.