Detection of phosphorylation post-translational modifications along single peptides with nanopores

Journal article (2023)

Authors

I.C. Nova BN/Cees Dekker Lab - AS , Kavli institute of nanoscience Delft
J. Ritmejeris BN/Cees Dekker Lab - AS , Kavli institute of nanoscience Delft
H.D. Brinkerhoff BN/Cees Dekker Lab - AS , Kavli institute of nanoscience Delft, University of Washington
T.J.R. Koenig BN/Bionanoscience , BN/Cees Dekker Lab - AS , Kavli institute of nanoscience Delft
Jens H. Gundlach University of Washington
C. Dekker BN/Cees Dekker Lab - AS , Kavli institute of nanoscience Delft
Research Group
BN/Cees Dekker Lab (AS) (TU Delft)
Copyright: © 2023 I.C. Nova, J. Ritmejeris, H.D. Brinkerhoff, T.J.R. Koenig, Jens H. Gundlach, C. Dekker
DOI
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https://doi.org/10.1038/s41587-023-01839-z
More Info
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Published Date

2023

Language

English

Reuse Rights

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Faculty

Applied Sciences

Department

BN/Bionanoscience

Research Group

BN/Cees Dekker Lab

Abstract

Current methods to detect post-translational modifications of proteins, such as phosphate groups, cannot measure single molecules or differentiate between closely spaced phosphorylation sites. We detect post-translational modifications at the single-molecule level on immunopeptide sequences with cancer-associated phosphate variants by controllably drawing the peptide through the sensing region of a nanopore. We discriminate peptide sequences with one or two closely spaced phosphates with 95% accuracy for individual reads of single molecules.