pH-gradient chromatofocusing of proteins on a chip

Conference paper (2015)

Authors

Hoon Suk Rho University of Twente
A.T. Hanke BT/Bioprocess Engineering - AS
M. Ottens BT/Bioprocess Engineering - AS
Han Gardeniers University of Twente
Research Group
BT/Bioprocess Engineering (AS) (TU Delft)
Microfluidics Chromatofocusing Micro-column PH gradient
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Published Date

2015

Language

English

Reuse Rights

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Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Bioprocess Engineering

Abstract

We present a novel microfluidic system for the pH-gradient focusing of proteins with the integration of 16 parallel micro-mixers, a micro-column, and a multiplexer. In this work we successfully achieved the creation of 16 non-linear gradients and the generation of a solid-phase micro-column for the realization of anion exchange chromatography on a single chip. With the device we demonstrated the separation of a protein mixture of R-phycoerythrin and FITC-BSA based on pH-gradient chromatofocusing.

Files

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