pH-gradient chromatofocusing of proteins on a chip

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pH-gradient chromatofocusing of proteins on a chip

Conference Paper (2015)

Author(s)

Hoon Suk Rho (University of Twente)

Alexander T. Hanke (TU Delft - BT/Bioprocess Engineering)

Marcel Ottens (TU Delft - BT/Bioprocess Engineering)

Han Gardeniers (University of Twente)

Research Group

BT/Bioprocess Engineering

Microfluidics Chromatofocusing Micro-column PH gradient

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https://resolver.tudelft.nl/uuid:1991ba92-8668-48c4-8b2f-b0e97cdde621

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Publication Year

2015

Language

English

Research Group

BT/Bioprocess Engineering

Pages (from-to)

245-247

ISBN (electronic)

9780979806483

Event

19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 (2015-10-25 - 2015-10-29), Gyeongju, Korea, Republic of

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Abstract

We present a novel microfluidic system for the pH-gradient focusing of proteins with the integration of 16 parallel micro-mixers, a micro-column, and a multiplexer. In this work we successfully achieved the creation of 16 non-linear gradients and the generation of a solid-phase micro-column for the realization of anion exchange chromatography on a single chip. With the device we demonstrated the separation of a protein mixture of R-phycoerythrin and FITC-BSA based on pH-gradient chromatofocusing.

External Files

http://www.rsc.org/images/LOC/2015/PDFs/Papers/0245_3A3-2.pdf Final published version