Electrochemical detection of enzyme kinetics using a nanofluidic thin layer cell device.

Conference paper (2010)

Authors

ED Goluch BN/Serge Lemay Lab - AS
N Wongrajit External organisation
PS Singh BN/Serge Lemay Lab - AS
AWJW Tepper External organisation
HA Heering External organisation
GW Canters External organisation
SG Lemay BN/Serge Lemay Lab - AS
Research Group
BN/Serge Lemay Lab (AS) (TU Delft)
More Info
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Published Date

2010

Language

English

Reuse Rights

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Faculty

Applied Sciences

Department

BN/Bionanoscience

Research Group

BN/Serge Lemay Lab

Abstract

ABSTRACT
We have developed a lithography-based nanofluidic device for sensitive electrochemical detection applications. The
device consists of a solution-filled channel bounded by parallel electrodes that are separated by ~50 nm. Redox cycling
at the two electrodes is used to amplify the electrochemical signals from molecules in the volume between the electrodes,
and can lead to dramatic improvements in sensitivity and/or selectivity. Interestingly, an important limit to sensitivity is
placed by statistical fluctuations in the number of molecules present in the device, a consequence of its extremely small
volume (a few femtolitres). In this Proceedings article, we focus on the consequences of these statistical fluctuations on
the signal-to-noise ratio of electrochemical sensors based on redox cycling.
KEYWORDS: Nanofluidics, electrochemistry, sensing, number fluctuations, mesoscopic.