Hydroxynitrile lyases covalently immobilized in continuous flow microreactors

Journal Article (2019)
Authors

Michelle P. van der Helm (TU Delft - ChemE/Advanced Soft Matter, Silesian University of Technology)

Paula Bracco Garcia (TU Delft - BT/Biocatalysis)

Hanna Busch (TU Delft - BT/Biocatalysis)

K. Szymańska (Silesian University of Technology)

A. Jarzȩbski (Polish Academy of Sciences, Silesian University of Technology)

U Hanefeld (TU Delft - BT/Biotechnologie)

Research Group
ChemE/Advanced Soft Matter
Copyright
© 2019 M. van der Helm, M.P. Bracco Garcia, H. Busch, Katarzyna Szymańska, Andrzej B. Jarzȩbski, U. Hanefeld
To reference this document use:
https://doi.org/10.1039/c8cy02192a
More Info
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Publication Year
2019
Language
English
Copyright
© 2019 M. van der Helm, M.P. Bracco Garcia, H. Busch, Katarzyna Szymańska, Andrzej B. Jarzȩbski, U. Hanefeld
Research Group
ChemE/Advanced Soft Matter
Issue number
5
Volume number
9
Pages (from-to)
1189-1200
DOI:
https://doi.org/10.1039/c8cy02192a
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Abstract


Enzymes are supreme catalysts when it comes to high enantiopurities and their immobilization will pave the way for continuous operation. In this context, we show the covalent immobilization of hydroxynitrile lyases HbHNL (from Hevea brasiliensis) and MeHNL (from Manihot esculenta) in a siliceous monolithic microreactor for continuous operation. A thorough characterization of the immobilized HNLs on mesoporous silicates indicated the conditions essential for a successful immobilization. Their application in a continuous flow system enabled a remarkably fast (3.2 min) production of chiral cyanohydrins with high conversion (97%) and high ee (98%) using minimal enzyme loading (STY = 71 g L
-1
h
-1
mg
protein

-1
). MeHNL showed increased operational stability, possibly due to a structural difference. The continuous flow microreactor outperformed batch systems, demonstrating the advantage of the mesoporous/macroporous environment for the expression of enzyme activity and the favorable characteristics of the microreactor. Overall, the system shows great potential for future industrial application of biocatalytic asymmetric syntheses.