A PLETHORA-auxin transcription module controls cell division plane rotation through MAP65 and CLASP

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A PLETHORA-auxin transcription module controls cell division plane rotation through MAP65 and CLASP

Journal Article (2012)

Author(s)

Pankaj Dhonukshe (Universiteit Utrecht)

Daan A. Weits (Universiteit Utrecht)

Alfredo Cruz-Ramirez (Universiteit Utrecht)

Eva E. Deinum (AMOLF Institute for Atomic and Molecular Physics)

Simon H. Tindemans (Imperial College London, AMOLF Institute for Atomic and Molecular Physics)

Klementina Kakar (Universiteit Utrecht)

Kalika Prasad (Universiteit Utrecht, Indian Institute of Science Education and Research Thiruvananthapuram)

Ari Pekka Mähönen (Universiteit Utrecht, University of Helsinki)

Chris Ambrose (University of British Columbia)

Michiko Sasabe (Nagoya University)

Guy Wachsmann (Universiteit Utrecht)

Marijn Luijten (Universiteit Utrecht)

Tom Bennett (Universiteit Utrecht, University of Cambridge)

Yasunori MacHida (Nagoya University)

Renze Heidstra (Universiteit Utrecht)

Geoffrey Wasteneys (University of British Columbia)

Bela M. Mulder (AMOLF Institute for Atomic and Molecular Physics, Wageningen University & Research)

Ben Scheres (Universiteit Utrecht)

Affiliation

External organisation

DOI related publication

https://doi.org/10.1016/j.cell.2012.02.051 Final published version

To reference this document use

https://resolver.tudelft.nl/uuid:2e152c3f-bd77-4073-a185-dfc482787bf3

More Info

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Publication Year

2012

Language

English

Affiliation

External organisation

Journal title

Cell

Issue number

2

Volume number

149

Pages (from-to)

383-396

Downloads counter

244

Abstract

Despite their pivotal role in plant development, control mechanisms for oriented cell divisions have remained elusive. Here, we describe how a precisely regulated cell division orientation switch in an Arabidopsis stem cell is controlled by upstream patterning factors. We show that the stem cell regulatory PLETHORA transcription factors induce division plane reorientation by local activation of auxin signaling, culminating in enhanced expression of the microtubule-associated MAP65 proteins. MAP65 upregulation is sufficient to reorient the cortical microtubular array through a CLASP microtubule-cell cortex interaction mediator-dependent mechanism. CLASP differentially localizes to cell faces in a microtubule- and MAP65-dependent manner. Computational simulations clarify how precise 90° switches in cell division planes can follow self-organizing properties of the microtubule array in combination with biases in CLASP localization. Our work demonstrates how transcription factor-mediated processes regulate the cellular machinery to control orientation of formative cell divisions in plants. PaperFlick: