Single objective tilted lightsheet for three-dimensional localization microscopy

Conference Paper (2021)
Author(s)

Shih Han Hung (TU Delft - Team Carlas Smith)

Jelmer Cnossen (TU Delft - Team Carlas Smith)

D. FAN (TU Delft - Team Carlas Smith)

S Smith (TU Delft - ImPhys/Computational Imaging, TU Delft - Team Carlas Smith)

Research Group
Team Carlas Smith
Copyright
© 2021 S. Hung, J.P. Cnossen, D. Fan, C.S. Smith
DOI related publication
https://doi.org/10.1117/12.2615803
More Info
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Publication Year
2021
Language
English
Copyright
© 2021 S. Hung, J.P. Cnossen, D. Fan, C.S. Smith
Research Group
Team Carlas Smith
ISBN (electronic)
9781510647107
Reuse Rights

Other than for strictly personal use, it is not permitted to download, forward or distribute the text or part of it, without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license such as Creative Commons.

Abstract

Optical sectioning technologies achieve high precision localization by reducing the background photon count. We use tilted light-sheet microscopy to achieve optical sectioning in localization microscopy, enabling thick sample observation and low background photon count images. A deformable mirror was incorporated to generate a tetrapod point spread function (PSF), enabling high resolution 3D localization. DNA-PAINT was imaged with 15 nm transverse and 60 nm axial resolution.

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