Cytochrome bd Displays Significant Quinol Peroxidase Activity

Journal Article (2016)
Author(s)

S. Al-Attar (TU Delft - BT/Biocatalysis)

Y. Yu (TU Delft - OLD BT/Analytical Biotechnology)

Martijn W.H. Pinkse (TU Delft - OLD BT/Analytical Biotechnology)

Jo Hoeser (Albert-Ludwigs-Universität Freiburg)

Thorsten Friedrich (Albert-Ludwigs-Universität Freiburg)

Dirk Bald (Vrije Universiteit Amsterdam)

S de Vries (TU Delft - BT/Biocatalysis)

Research Group
BT/Biocatalysis
Copyright
© 2016 S. Al-Attar, Y. Yu, M.W.H. Pinkse, Jo Hoeser, Thorsten Friedrich, Dirk Bald, S. de Vries
DOI related publication
https://doi.org/10.1038/srep27631
More Info
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Publication Year
2016
Language
English
Copyright
© 2016 S. Al-Attar, Y. Yu, M.W.H. Pinkse, Jo Hoeser, Thorsten Friedrich, Dirk Bald, S. de Vries
Research Group
BT/Biocatalysis
Volume number
6
Reuse Rights

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Abstract

Cytochrome bd is a prokaryotic terminal oxidase that catalyses the electrogenic reduction of oxygen to water using ubiquinol as electron donor. Cytochrome bd is a tri-haem integral membrane enzyme carrying a low-spin haem b558, and two high-spin haems: b595 and d. Here we show that besides its oxidase activity, cytochrome bd from Escherichia coli is a genuine quinol peroxidase (QPO) that reduces hydrogen peroxide to water. The highly active and pure enzyme preparation used in this study did not display the catalase activity recently reported for E. coli cytochrome bd. To our knowledge, cytochrome bd is the first membrane-bound quinol peroxidase detected in E. coli. The observation that cytochrome bd is a quinol peroxidase, can provide a biochemical basis for its role in detoxification of hydrogen peroxide and may explain the frequent findings reported in the literature that indicate increased sensitivity to hydrogen peroxide and decreased virulence in mutants that lack the enzyme.