Model based wavefront sensorless adaptive optics in confocal microscopy with reflective pinhole
P. Pozzi (TU Delft - Team Raf Van de Plas)
Oleg Soloviev (ITMO University, TU Delft - Team Raf Van de Plas, Flexible Optical B.V.)
D. Wilding (TU Delft - Team Raf Van de Plas)
Gleb Vdovin (Flexible Optical B.V., TU Delft - Team Raf Van de Plas, ITMO University)
M.H.G. Verhaegen (TU Delft - Team Raf Van de Plas)
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Abstract
We present a new method for fast wavefront sensorless correction of phase aberrations in confocal microscopy, based on a physical model of the distribution of fluorescence light rejected by the pinhole. The method is described, and an experimental confirmation of the method is provided.
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