Enzyme engineering for biocatalysis

Journal Article (2024)
Author(s)

CE Paul (TU Delft - BT/Biocatalysis)

U Hanefeld (TU Delft - BT/Biocatalysis)

F Hollmann (TU Delft - BT/Biocatalysis)

Ge Qu (Chinese Academy of Sciences)

Bo Yuan (Chinese Academy of Sciences)

Zhoutong Sun (Chinese Academy of Sciences)

Research Group
BT/Biocatalysis
Copyright
© 2024 C.E. Paul, U. Hanefeld, F. Hollmann, Ge Qu, Bo Yuan, Zhoutong Sun
DOI related publication
https://doi.org/10.1016/j.mcat.2024.113874
More Info
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Publication Year
2024
Language
English
Copyright
© 2024 C.E. Paul, U. Hanefeld, F. Hollmann, Ge Qu, Bo Yuan, Zhoutong Sun
Research Group
BT/Biocatalysis
Volume number
555
Reuse Rights

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Abstract

Contemporary Biocatalysis heavily relies on enzyme engineering as natural enzymes frequently lack the requisite attributes for effective organic synthesis. The inherent limitations in stability, catalytic activity, and selectivity of wild-type enzymes often hinder their suitability for chemical synthesis. Over the past 25 years, there has been an unprecedented advancement in protein engineering tools, empowering enzymologists to customise enzymes to precisely meet the demands of organic synthesis. In this discussion, we delineate some of the most crucial techniques in enzyme engineering and their significance in facilitating chemical synthesis.