Engineering a lipase B from Candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity
Xu Wang (South China University of Technology)
Peng Fei Zhou (South China University of Technology)
Gang Li (South China University of Technology)
Bo Yang (South China University of Technology)
F Hollmann (TU Delft - BT/Biocatalysis)
Yong Hua Wang (South China University of Technology)
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Abstract
A Ser105Ala mutant of the lipase B from Candida antarctica enables 'perhydrolase-only' reactions. At the example of the chemoenzymatic Baeyer-Villiger oxidation of cyclohexanone, we demonstrate that with this mutant selective oxidation can be achieved in deep eutectic solvent while essentially eliminating the undesired hydrolysis reaction of the product.
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