Automated Evolutionary Engineering of Yeasts
Erik A.F. de Hulster (TU Delft - BT/Industriele Microbiologie)
C. Mooiman (TU Delft - BT/Bioprocess Engineering)
Rowin Timmermans (Applikon Biotechnology)
R. Mans (TU Delft - BT/Industriele Microbiologie)
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Abstract
Evolutionary engineering of microbes provides a powerful tool for untargeted optimization of (engineered) cell factories and identification of genetic targets for further research. Directed evolution is an intrinsically time-intensive effort, and automated methods can significantly reduce manual labor. Here, design considerations for various evolutionary engineering methods are described, and generic workflows for batch-, chemostat-, and accelerostat-based evolution in automated bioreactors are provided. These methods can be used to evolve yeast cultures for >1000 generations and are designed to require minimal manual intervention.