Biocatalytic reduction of alkenes in micro-aqueous organic solvent catalysed by an immobilised ene reductase

Journal article (2023)

Authors

R. Villa Aroca BT/Biocatalysis - AS
C. Ferrer Carbonell BT/Biocatalysis - AS
C.E. Paul BT/Biocatalysis - AS
Research Group
BT/Biocatalysis (AS) (TU Delft)
Copyright: © 2023 R. Villa Aroca, C. Ferrer Carbonell, C.E. Paul
DOI: https://doi.org/10.1039/d3cy00541k
More Info
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Published Date

2023

Language

English

Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Biocatalysis

Abstract

Biocatalytic asymmetric reduction of alkenes in organic solvent is attractive for enantiopurity and product isolation, yet remains under developed. Herein we demonstrate the robustness of an ene reductase immobilised on Celite for the reduction of activated alkenes in micro-aqueous organic solvent. Full conversion was obtained in methyl t-butyl ether, avoiding hydrolysis and racemisation of products. The immobilised ene reductase showed reusability and a scale-up demonstrated its applicability.