Quantitative measurement and visualization of biofilm O 2 consumption rates in membrane filtration systems

Journal article (2012)

Authors

E.I.E.D. Prest BT/Environmental Biotechnology - AS , Centre of Excellence for Sustainable Water Technology, Wetsus, European Centre of Excellence for Sustainable Water Technology
M.J. Staal Marine Biological Section, BT/Environmental Biotechnology - AS , University of Copenhagen
M Kuhl Nanyang Technological University, University of Copenhagen, University of Technology Sydney
Mark C.M. van Loosdrecht BT/Environmental Biotechnology - AS
J.S. Vrouwenvelder Water Desalination and Reuse Center, Centre of Excellence for Sustainable Water Technology, King Abdullah University of Science and Technology, BT/Environmental Biotechnology - AS , Wetsus, European Centre of Excellence for Sustainable Water Technology
Research Group
BT/Environmental Biotechnology (AS) (TU Delft)
Biological activity measurement Concentration polarization Non-destructive biofouling diagnosis Flow channels Optode
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Published Date

01-03-2012

Language

English

Reuse Rights

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Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Environmental Biotechnology

Abstract

There is a strong need for techniques enabling direct assessment of biological activity of biofouling in membrane filtration systems. Here we present a new quantitative and non-destructive method for mapping O 2 dynamics in biofilms during biofouling studies in membrane fouling simulators (MFS). Transparent planar O 2 optodes in combination with a luminescence lifetime imaging system were used to map the two-dimensional distribution of O 2 concentrations and consumption rates inside the MFS. The O 2 distribution was indicative for biofilm development. Biofilm activity was characterized by imaging of O 2 consumption rates, where low and high activity areas could be clearly distinguished. The spatial development of O 2 consumption rates, flow channels and stagnant areas could be determined. This can be used for studies on concentration polarization, i.e. salt accumulation at the membrane surface resulting in increased salt passage and reduced water flux. The new optode-based O 2 imaging technique applied to MFS allows non-destructive and spatially resolved quantitative biological activity measurements (BAM) for on-site biofouling diagnosis and laboratory studies. The following set of complementary tools is now available to study development and control of biofouling in membrane systems: (i) MFS, (ii) sensitive pressure drop measurement, (iii) magnetic resonance imaging, (iv) numerical modelling, and (v) biological activity measurement based on O 2 imaging methodology.