Bacterial Outer Membrane Vesicles as Nano-Scale Bioreactors
A Fatty Acid Conversion Case Study
Ji Won Song (Ewha Womans University)
Yoonjin Baeg (Ewha Womans University)
Ha Yeon Jeong (Ewha Womans University)
Jinwon Lee (Sogang University)
Deok Kun Oh (Konkuk University, Seoul )
Frank Hollmann (TU Delft - BT/Biocatalysis)
Jin Byung Park (Ewha Womans University)
More Info
expand_more
Other than for strictly personal use, it is not permitted to download, forward or distribute the text or part of it, without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license such as Creative Commons.
Abstract
Bacterial outer membrane vesicles (OMVs) are small unilamellar proteoliposomes, involved in various functions including cell-to-cell signalling and protein excretion. We have engineered the OMVs of Escherichia coli to nano-scaled bioreactors for the biotransformation of fatty acids by targeting a fatty acid double bond hydratase of Stentrophomonas maltophilia (SmOhyA) and/or a photoactivated fatty acid decarboxylase from Chlorella variabilis NC64 A (CvFAP) into OMVs. Engineered OMVs containing both SmOhyA and CvFAP were able to catalyse the transformation of oleic acid ((Z)-octadec-9-enoic acid) into 9-hydroxyheptadecane via (R)-10-hydroxyoctadecanoic acid. The specific biotransformation rates of oleic acid reached 8.0×10−12 μmol/min per OMV.