Bacterial Outer Membrane Vesicles as Nano-Scale Bioreactors

A Fatty Acid Conversion Case Study

Journal Article (2021)
Author(s)

Ji Won Song (Ewha Womans University)

Yoonjin Baeg (Ewha Womans University)

Ha Yeon Jeong (Ewha Womans University)

Jinwon Lee (Sogang University)

Deok Kun Oh (Konkuk University, Seoul )

Frank Hollmann (TU Delft - BT/Biocatalysis)

Jin Byung Park (Ewha Womans University)

Research Group
BT/Biocatalysis
Copyright
© 2021 Ji Won Song, Yoonjin Baeg, Ha Yeon Jeong, Jinwon Lee, Deok Kun Oh, F. Hollmann, Jin Byung Park
DOI related publication
https://doi.org/10.1002/cctc.202100778
More Info
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Publication Year
2021
Language
English
Copyright
© 2021 Ji Won Song, Yoonjin Baeg, Ha Yeon Jeong, Jinwon Lee, Deok Kun Oh, F. Hollmann, Jin Byung Park
Research Group
BT/Biocatalysis
Issue number
19
Volume number
13
Pages (from-to)
4080-4086
Reuse Rights

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Abstract

Bacterial outer membrane vesicles (OMVs) are small unilamellar proteoliposomes, involved in various functions including cell-to-cell signalling and protein excretion. We have engineered the OMVs of Escherichia coli to nano-scaled bioreactors for the biotransformation of fatty acids by targeting a fatty acid double bond hydratase of Stentrophomonas maltophilia (SmOhyA) and/or a photoactivated fatty acid decarboxylase from Chlorella variabilis NC64 A (CvFAP) into OMVs. Engineered OMVs containing both SmOhyA and CvFAP were able to catalyse the transformation of oleic acid ((Z)-octadec-9-enoic acid) into 9-hydroxyheptadecane via (R)-10-hydroxyoctadecanoic acid. The specific biotransformation rates of oleic acid reached 8.0×10−12 μmol/min per OMV.