Correlating 3D light to 3D electron microscopy for systems biology

Journal article (2017)

Authors

LM Collinson Francis Crick Institute
E.C.M. Carroll ImPhys/Charged Particle Optics
J.P. Hoogenboom ImPhys/Charged Particle Optics
Research Group
ImPhys/Charged Particle Optics
Copyright: © 2017 LM Collinson, E.C.M. Carroll, J.P. Hoogenboom
DOI: https://doi.org/10.1016/j.cobme.2017.10.006
Systems biology Correlative microscopy 3D CLEM Large scale imaging Volume electron microscopy Ultrastructure
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Published Date

15-11-2017

Language

English

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Research Group

ImPhys/Charged Particle Optics

Abstract

Whilst a ‘resolution revolution’ has taken place at the macromolecular scale in both electron microscopy and light microscopy, a ‘volume revolution’ has taken place at the tissue and organism level in both imaging modalities. At both ends of the scale – resolution and volume – there are concerted efforts to link the information from light and electron microscopes through correlative workflows to link structure to function. Here, we consider the status and potential of correlative imaging in the volume domain (3D CLEM).