Correlating 3D light to 3D electron microscopy for systems biology
LM Collinson (Francis Crick Institute)
E.C.M. Carroll (TU Delft - ImPhys/Charged Particle Optics)
J.P. Hoogenboom (TU Delft - ImPhys/Charged Particle Optics)
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Abstract
Whilst a ‘resolution revolution’ has taken place at the macromolecular scale in both electron microscopy and light microscopy, a ‘volume revolution’ has taken place at the tissue and organism level in both imaging modalities. At both ends of the scale – resolution and volume – there are concerted efforts to link the information from light and electron microscopes through correlative workflows to link structure to function. Here, we consider the status and potential of correlative imaging in the volume domain (3D CLEM).