Immobilization and Application of Fatty Acid Photodecarboxylase in Deep Eutectic Solvents
Santiago Nahuel Chanquia (Aarhus University)
Frederik Vig Benfeldt (Aarhus University)
Noémi Petrovai (Aarhus University)
Paul Santner (Aarhus University)
Frank Hollmann (TU Delft - BT/Biocatalysis)
Bekir Engin Eser (Aarhus University)
Selin Kara (Aarhus University, Leibniz Universität)
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Abstract
Since its discovery in 2017, the fatty acid decarboxylase (FAP) photoenzyme has been the focus of extensive research, given its ability to convert fatty acids into alka(e)nes using merely visible blue light. Unfortunately, there are still some drawbacks that limit the applicability of this biocatalyst, such as poor solubility of the substrates in aqueous media, poor photostability, and the impossibility of reusing the catalyst for several cycles. In this work, we demonstrate the use of FAP in non-conventional media as a free enzyme and an immobilized preparation. Namely, its applicability in deep eutectic solvents (DESs) and a proof-of-concept immobilization using a commercial His-tag selective carrier, a thorough study of reaction and immobilization conditions in each case, as well as reusability studies are shown. We observed an almost complete selectivity of the enzyme towards C18 decarboxylation over C16 when used in a DES, with a product analytical yield up to 81 % when using whole cells. Furthermore, when applying the immobilized enzyme in DES, we obtained yields >10-fold higher than the ones obtained in aqueous media.
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