E. coli Nickel-Iron Hydrogenase 1 Catalyses Non-native Reduction of Flavins
Demonstration for Alkene Hydrogenation by Old Yellow Enzyme Ene-reductases**
Shiny Joseph Srinivasan (University of Oxford)
Sarah E. Cleary (University of Oxford)
Miguel A. Ramirez (University of Oxford)
Holly A. Reeve (University of Oxford)
Caroline E. Paul (TU Delft - BT/Biocatalysis)
Kylie A. Vincent (University of Oxford)
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Abstract
A new activity for the [NiFe] uptake hydrogenase 1 of Escherichia coli (Hyd1) is presented. Direct reduction of biological flavin cofactors FMN and FAD is achieved using H2 as a simple, completely atom-economical reductant. The robust nature of Hyd1 is exploited for flavin reduction across a broad range of temperatures (25–70 °C) and extended reaction times. The utility of this system as a simple, easy to implement FMNH2 or FADH2 regenerating system is then demonstrated by supplying reduced flavin to Old Yellow Enzyme “ene-reductases” to support asymmetric alkene reductions with up to 100 % conversion. Hyd1 turnover frequencies up to 20.4 min−1 and total turnover numbers up to 20 200 were recorded during flavin recycling.
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