Optimal model-based sensorless adaptive optics for epifluorescence microscopy
Paolo Pozzi (TU Delft - Mechanical Engineering)
Oleg Soloviev (Flexible Optical B.V., TU Delft - Mechanical Engineering, ITMO University)
Dean Wilding (TU Delft - Mechanical Engineering)
Gleb Vdovin (TU Delft - Mechanical Engineering, Flexible Optical B.V., ITMO University)
Michel Verhaegen (TU Delft - Mechanical Engineering)
More Info
expand_more
Other than for strictly personal use, it is not permitted to download, forward or distribute the text or part of it, without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license such as Creative Commons.
Abstract
We report on a universal sample-independent sensorless adaptive optics method, based on modal optimization of the second moment of the fluorescence emission from a point-like excitation. Our method employs a sample-independent precalibration, performed only once for the particular system, to establish the direct relation between the image quality and the aberration. The method is potentially applicable to any form of microscopy with epifluorescence detection, including the practically important case of incoherent fluorescence emission from a three dimensional object, through minor hardware modifications. We have applied the technique successfully to a widefield epifluorescence microscope and to a multiaperture confocal microscope.
help_outline