Electrotransfer for nucleic acid and protein delivery
A. Muralidharan (TU Delft - BN/Stan Brouns Lab, Kavli institute of nanoscience Delft)
P. Boukany (TU Delft - ChemE/Product and Process Engineering)
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Abstract
Electrotransfer of nucleic acids and proteins has become crucial in biotechnology for gene augmentation and genome editing. This review explores the applications of electrotransfer in both ex vivo and in vivo scenarios, emphasizing biomedical uses. We provide insights into completed clinical trials and successful instances of nucleic acid and protein electrotransfer into therapeutically relevant cells such as immune cells and stem and progenitor cells. In addition, we delve into emerging areas of electrotransfer where nanotechnology and deep learning techniques overcome the limitations of traditional electroporation.
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