Cellular Tango
How extracellular matrix adhesion choreographs Rac-Rho signaling and cell movement
E.G. Rens (TU Delft - Mathematical Physics, University of British Columbia)
Leah Edelstein-Keshet (University of British Columbia)
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Abstract
The small GTPases Rac and Rho are known to regulate eukaryotic cell shape, promoting front protrusion (Rac) or rear retraction (Rho) of the cell edge. Such cell deformation changes the contact and adhesion of cell to the extracellular matrix (ECM), while ECM signaling through integrin receptors also affects GTPase activity. We develop and investigate a model for this three-way feedback loop in 1D and 2D spatial domains, as well as in a fully deforming 2D cell shapes with detailed adhesion-bond biophysics. The model consists of reaction-diffusion equations solved numerically with open-source software, Morpheus, and with custom-built cellular Potts model simulations. We find a variety of patterns and cell behaviors, including persistent polarity, flipped front-back cell polarity oscillations, spiral waves, and random protrusion-retraction.We show that the observed spatial patterns depend on the cell shape, and vice versa.
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