Single objective tilted lightsheet for three-dimensional localization microscopy

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Single objective tilted lightsheet for three-dimensional localization microscopy

Conference Paper (2021)

Author(s)

Shih Te Hung (TU Delft - Mechanical Engineering)

Jelmer Cnossen (TU Delft - Mechanical Engineering)

Daniel Fan (TU Delft - Mechanical Engineering)

Carlas S. Smith (TU Delft - Mechanical Engineering)

Research Group

Team Carlas Smith

URL related publication

https://www.osapublishing.org/abstract.cfm?uri=ECBO-2021-EM1A.37 Final published version

To reference this document use

https://resolver.tudelft.nl/uuid:63ec8c6b-016c-47ef-9d20-1df2bb16bc4e

More Info

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Publication Year

2021

Language

English

Research Group

Team Carlas Smith

Article number

EM1A.37

ISBN (print)

978-1-943580-95-8

ISBN (electronic)

978-1-557528-20-9

Event

2021 European Conferences on Biomedical Optics, ECBO 2021 (2021-06-20 - 2021-06-24), Virtual, Online, Germany

Downloads counter

235

Abstract

Optical sectioning technologies achieve high precision localization by reducing the background photon count. We use tilted light-sheet microscopy to achieve optical sectioning in localization microscopy, enabling thick sample observation and low background photon count images. A deformable mirror was incorporated to generate a tetrapod point spread function (PSF), enabling high resolution 3D localization. DNA-PAINT was imaged with 15 nm transverse and 60 nm axial resolution.