Electochemical detection of enzyme kinetics using a nanofluidic thin layer cell device.

Conference paper (2010)

Authors

ED Goluch BN/Serge Lemay Lab - AS
N Wongrajit External organisation
PS Singh BN/Serge Lemay Lab - AS
AWJW Tepper External organisation
HA Heering External organisation
GW Canters External organisation
SG Lemay BN/Serge Lemay Lab - AS
Research Group
BN/Serge Lemay Lab (AS) (TU Delft)
More Info
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Published Date

2010

Language

English

Reuse Rights

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Faculty

Applied Sciences

Department

BN/Bionanoscience

Research Group

BN/Serge Lemay Lab

Abstract

ABSTRACT
Amperometric detection is an attractive detection scheme for mico- and nanofluidic systems as it directly yields an
electrical signal. However, this scheme is not often utilized because of its limited sensitivity. We overcome this limitation
by employing a nanofluidic device consisting of a solution-filled cavity bounded by two closely spaced parallel electrodes
that can amplify the current by repeatedly reducing and oxidizing electroactive species. Here we demonstrate the
utility of such a device to measure enzyme kinetics in real time. We compare the conversion of p-cresol into methylquinone
by tyrosinase using our nanofluidic device against a standard UV-Vis spectroscopic protocol.
KEYWORDS: Electrochemistry, Redox Cycling, Enzyme Kinetics, Nanofluidics