Live-cell single-vRNP imaging identifies viral gene expression signatures that shape influenza infection heterogeneity
Huib H. Rabouw ( University Medical Centre Utrecht, Oncode Institute, Hubrecht Institute)
Janin Schokolowski (Oncode Institute, Hubrecht Institute, University Medical Centre Utrecht)
Micha Müller (Oncode Institute, Hubrecht Institute, University Medical Centre Utrecht)
Matthijs J.D. Baars (Oncode Institute, Hubrecht Institute, University Medical Centre Utrecht)
Antonella F.M. Dost ( University Medical Centre Utrecht, Hubrecht Institute, Oncode Institute)
Theo M. Bestebroer (Erasmus MC)
Jakob Püschel ( University Medical Centre Utrecht, Hubrecht Institute, Oncode Institute)
Hans Clevers (Roche Innovation Center, University Medical Centre Utrecht, Oncode Institute, Hubrecht Institute)
Ron A.M. Fouchier (Erasmus MC)
Marvin E. Tanenbaum (Oncode Institute, University Medical Centre Utrecht, TU Delft - BN/Marvin Tanenbaum Lab, Hubrecht Institute)
More Info
expand_more
Other than for strictly personal use, it is not permitted to download, forward or distribute the text or part of it, without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license such as Creative Commons.
Abstract
Cell-to-cell heterogeneity in infection outcome is a general feature of most viruses, but the underlying mechanisms are poorly understood. Here, we developed a live-cell single-molecule imaging technology to visualize infection by unmodified influenza A viruses (IAVs) with unprecedented resolution. Using this approach, we generated a detailed kinetic map of IAV infection, which identified viral ribonucleoprotein (vRNP) replication, nuclear export, and virion budding as important sources of heterogeneity. Mechanistically, we show that infection heterogeneity is caused by differential viral gene expression signatures, resulting from widespread transcriptional defects and loss of viral genome segments. For example, loss of NS, but surprisingly not polymerase subunits, severely delays replication onset, and loss of M and NS, but not HA, underlies vRNP nuclear export defects. In summary, our work identifies the origin and consequences of infection heterogeneity and provides a broadly applicable technology that allows high-resolution phenotyping of unmodified IAVs and other negative-strand RNA viruses.
help_outline