Structure Guided Directed Evolution of Enzymes
Sean Hüppi (TU Delft - BT/Biocatalysis)
F. Hollmann – Promotor (TU Delft - BT/Biocatalysis)
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Abstract
Our ability to tailor enzymatic properties is a critical factor for biocatalyst application in the industrial sector. Although many wild-type enzymes have been found capable of promiscuously catalysing desired anthropogenic reactions, their activity and selectivity for non-natural transformations is often poor. Consequently, it is crucial to optimise enzymes such that they can be effectively integrated into industrial processes. Notable added advantages in this context are that enzymes are considered 'green' catalysts - enhancing the perceived value of products in today's environmentally-conscious society – and that biocatalysts can carry out intricate chemistries with exceptional regio- and stereoselectivity, complementing traditional organic synthesis.
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