A hydrogen-driven biocatalytic approach to recycling synthetic analogues of NAD(P)H
Holly A. Reeve (University of Oxford)
Jake Nicholson (University of Oxford)
Farieha Altaf (University of Oxford)
Thomas H. Lonsdale (University of Oxford)
Janina Preissler (Technical University of Berlin)
Lars Lauterbach (Technical University of Berlin, RWTH Aachen University)
Oliver Lenz (Technical University of Berlin)
Silke Leimkühler (University of Potsdam)
F. Hollmann (TU Delft - BT/Biocatalysis)
C.E. Paul (TU Delft - BT/Biocatalysis)
Kylie A. Vincent (University of Oxford)
More Info
expand_more
Other than for strictly personal use, it is not permitted to download, forward or distribute the text or part of it, without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license such as Creative Commons.
Abstract
We demonstrate a recycling system for synthetic nicotinamide cofactor analogues using a soluble hydrogenase with turnover number of >1000 for reduction of the cofactor analogues by H2. Coupling this system to an ene reductase, we show quantitative conversion of N-ethylmaleimide to N-ethylsuccinimide. The biocatalyst system retained >50% activity after 7 h.
help_outline