Correlative polarized light imaging and two-photon fluorescence microscopy for 3D myelinated fibers reconstruction

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Correlative polarized light imaging and two-photon fluorescence microscopy for 3D myelinated fibers reconstruction

Conference Paper (2017)

Author(s)

Irene Costantini (University of Florence)

Miriam Menzel (Forschungszentrum Jülich)

Ludovico Silvestri (University of Florence)

Nicole Schubert (Forschungszentrum Jülich)

Markus Axer (Forschungszentrum Jülich)

Katrin Amunts (Universität Düsseldorf, Forschungszentrum Jülich)

Francesco S. Pavone (Istituto Nazionale di Ottica, Consiglio Nazionale delle Ricerche, University of Florence)

Affiliation

External organisation

DOI related publication

https://doi.org/10.1117/12.2284328 Final published version

To reference this document use

https://resolver.tudelft.nl/uuid:709db857-92c7-4c48-8e5b-7b31392f2d91

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Publication Year

2017

Language

English

Affiliation

External organisation

Article number

1041306

ISBN (electronic)

9781510612846

Event

Novel Biophotonics Techniques and Applications IV 2017 (2017-06-25 - 2017-06-27), Munich, Germany

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Abstract

In this work, we employ an integrated label-free dual approach that combines Polarized Light Imaging with Two-Photon Fluorescence Microscopy to study fiber orientations of myelinated axons in fixed brain slices from different species.