Correlative polarized light imaging and two-photon fluorescence microscopy for 3D myelinated fibers reconstruction

Conference paper (2017)

Authors

Irene Costantini University of Florence
Miriam Menzel Forschungszentrum Jülich
Ludovico Silvestri University of Florence
Nicole Schubert Forschungszentrum Jülich
Markus Axer Forschungszentrum Jülich
Katrin Amunts Forschungszentrum Jülich, Universität Düsseldorf
Francesco S. Pavone CNR-INO, University of Florence
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Published Date

2017

Language

English

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Abstract

In this work, we employ an integrated label-free dual approach that combines Polarized Light Imaging with Two-Photon Fluorescence Microscopy to study fiber orientations of myelinated axons in fixed brain slices from different species.