Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production

Journal article (2022)

Authors

A.C.A. van Aalst BT/Industriele Microbiologie - AS
S.C. de Valk BT/Industriele Microbiologie - AS
W.M. van Gulik BT/Industriele Microbiologie - AS
J.T. Pronk BT/Biotechnologie
R. Mans BT/Industriele Microbiologie - AS
Research Group
BT/Industriele Microbiologie (AS) (TU Delft)
Copyright: © 2022 A.C.A. van Aalst, S.C. de Valk, W.M. van Gulik, Mickel L.A. Jansen, J.T. Pronk, R. Mans
DOI
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https://doi.org/10.1016/j.synbio.2021.12.010
Saccharomyces cerevisiae Synthetic biology Biofuels Metabolic engineering Redox metabolism Energy metabolism
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Published Date

2022

Language

English

Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Industriele Microbiologie

Abstract

Product yield on carbohydrate feedstocks is a key performance indicator for industrial ethanol production with the yeast Saccharomyces cerevisiae. This paper reviews pathway engineering strategies for improving ethanol yield on glucose and/or sucrose in anaerobic cultures of this yeast by altering the ratio of ethanol production, yeast growth and glycerol formation. Particular attention is paid to strategies aimed at altering energy coupling of alcoholic fermentation and to strategies for altering redox-cofactor coupling in carbon and nitrogen metabolism that aim to reduce or eliminate the role of glycerol formation in anaerobic redox metabolism. In addition to providing an overview of scientific advances we discuss context dependency, theoretical impact and potential for industrial application of different proposed and developed strategies.