Mathematical modelling for the optimization of cellulase production using glycerol for cell growth and cellulose as the inducer substrate
L. Gelain (University of Campinas, TU Delft - OLD BT/Cell Systems Engineering)
Luuk A.M. Luuk (University of Limerick, TU Delft - BT/Bioprocess Engineering)
WM Van Gulik (TU Delft - OLD BT/Cell Systems Engineering)
José Geraldo Geraldo da Cruz Pradella (Universidade Federal de Sao Paulo)
Aline da Costa (University of Campinas)
More Info
expand_more
Other than for strictly personal use, it is not permitted to download, forward or distribute the text or part of it, without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license such as Creative Commons.
Abstract
Cellulase production can be divided into two steps: growth stage; followed by an induction stage. To develop a mathematical model for the optimization of this strategy, two sets of experiments were performed in batch mode for parameter estimation. One set of experiments was performed to evaluate the influence of glycerol regarding cell growth (initial concentrations of 5, 10, 15 and 20 g/L). The other set of experiments considered the induction stage using cellulose as the substrate (initial concentrations of 5, 10, 20, 30 and 40 g/L). Two feeding strategies were simulated to maximize cellulase production using glycerol to maintain a high cell concentration. The first simulation used a discrete feed and the second used a continuous feed of cellulose. The mathematical model proposed allows maintaining a high cell concentration and the addition of optimal small amounts of the inducer substrate to prevent inhibition of enzyme production.
help_outline