Natronogracilivirga saccharolytica gen. nov., sp. nov. and Cyclonatronum proteinivorum gen. nov., sp. nov., haloalkaliphilic organotrophic bacteroidetes from hypersaline soda lakes forming a new family Cyclonatronaceae fam. nov. in the order Balneolales

Journal article (2023)

Authors

Tatjana N. Zhilina Winogradsky Institute of Microbiology
Dimitry Y. Sorokin BT/Environmental Biotechnology - AS
Stepan V. Toschakov Kurchatov Center for Genome Research, National Research Center “Kurchatov Institute”,
Ilya V. Kublanov Lomonosov Moscow State University, Moscow, Winogradsky Institute of Microbiology
Daria G. Zavarzina Winogradsky Institute of Microbiology
Research Group
BT/Environmental Biotechnology (AS) (TU Delft)
Copyright: © 2023 Tatjana N. Zhilina, Dimitry Y. Sorokin, Stepan V. Toschakov, Ilya V. Kublanov, Daria G. Zavarzina
DOI
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https://doi.org/10.1016/j.syapm.2023.126403
Soda lakes Bacteroidota Cyclonatronum Natronogracilivirga Cyclonatronaceae Balneolales
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Published Date

2023

Language

English

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Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Environmental Biotechnology

Abstract

Two heterotrophic bacteroidetes strains were isolated as satellites from autotrophic enrichments inoculated with samples from hypersaline soda lakes in southwestern Siberia. Strain Z-1702
T is an obligate anaerobic fermentative saccharolytic bacterium from an iron-reducing enrichment culture, while Ca. Cyclonatronum proteinivorum Omega
T is an obligate aerobic proteolytic microorganism from a cyanobacterial enrichment. Cells of isolated bacteria are characterized by highly variable morphology. Both strains are chloride-independent moderate salt-tolerant obligate alkaliphiles and mesophiles. Strain Z-1702
T ferments glucose, maltose, fructose, mannose, sorbose, galactose, cellobiose, N-acetyl-glucosamine and alpha-glucans, including starch, glycogen, dextrin, and pullulan. Strain Omega
T is strictly proteolytic utilizing a range of proteins and peptones. The main polar lipid fatty acid in both strains is iso-C
15:0, while other major components are various C
16 and C
17 isomers. According to pairwise sequence alignments using BLAST Gracilimonas was the nearest cultured relative to both strains (<90% of 16S rRNA gene sequence identity). Phylogenetic analysis placed strain Z-1702
T and strain Omega
T as two different genera in a deep-branching clade of the new family level within the order Balneolales with genus. Based on physiological characteristics and phylogenetic position of strain Z-1702
T it was proposed to represent a novel genus and species Natronogracilivirga saccharolityca gen. nov., sp. nov. (= DSMZ 109061
T =JCM 32930
T =VKM B 3262
T). Furthermore, phylogenetic and phenotypic parameters of N. saccharolityca and C. proteinivorum gen. nov., sp. nov., strain Omega
T (=JCM 31662
T, =UNIQEM U979
T), make it possible to include them into a new family with a proposed designation Cyclonatronaceae fam. nov.