Pupil filters for extending the field-of-view in light-sheet microscopy
D. Wilding (TU Delft - Team Raf Van de Plas)
Paolo Pozzi (TU Delft - Team Raf Van de Plas)
O.A. Soloviev (Flexible Optical B.V., TU Delft - Team Raf Van de Plas)
Gleb Vdovin (TU Delft - Team Raf Van de Plas, Flexible Optical B.V.)
C.J. Sheppard (Italian Institute of Technology)
M.H.G. Verhaegen (TU Delft - Team Raf Van de Plas)
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Abstract
Pupil filters, represented by binary phase modulation, have been applied to extend the field of view of a light-sheet fluorescence microscope. Optimization has been used, first numerically to calculate the optimum filter structure and then experimentally, to scale and align the numerically synthesized filter in the microscope. A significant practical extension of the field of view has been observed, making the reported approach a valuable tool on the path to wide-field light-sheet microscopy.
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