Navigating the crowd
visualizing coordination between genome dynamics, structure, and transcription
Haitham A. Shaban (National Research Centre, Dokki, Cairo,, École Polytechnique Fédérale de Lausanne)
Roman Barth (TU Delft - BN/Cees Dekker Lab)
Kerstin Bystricky (CNRS-UPS)
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Abstract
The eukaryotic genome is hierarchically structured yet highly dynamic. Regulating transcription in this environment demands a high level of coordination to permit many proteins to interact with chromatin fiber at appropriate sites in a timely manner. We describe how recent advances in quantitative imaging techniques overcome caveats of sequencing-based methods (Hi-C and related) by enabling direct visualization of transcription factors and chromatin at high resolution, from single genes to the whole nucleus. We discuss the contribution of fluorescence imaging to deciphering the principles underlying this coordination within the crowded nuclear space in living cells and discuss challenges ahead.
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