Closed-loop optogenetic control of cell biology enables outcome-driven microscopy
Josiah B. Passmore (Universiteit Utrecht)
Alfredo Rates (Universiteit Utrecht)
Jakob Schröder (Universiteit Utrecht)
Menno T.P. van Laarhoven (TU Delft - Mechanical Engineering)
Vincent J.W. Hellebrekers (Universiteit Utrecht)
Henrik G. van Hoef (Universiteit Utrecht)
Antonius J.M. Geurts (Universiteit Utrecht)
Wendy van Straaten (Universiteit Utrecht)
Wilco Nijenhuis (Universiteit Utrecht)
Florian Berger (Universiteit Utrecht)
Carlas S. Smith (TU Delft - Mechanical Engineering)
Ihor Smal (Universiteit Utrecht)
Lukas C. Kapitein (Universiteit Utrecht)
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Abstract
Smart microscopy is transforming biological imaging by integrating real-time analysis with adaptive acquisition to enhance imaging efficiency. Whereas many emerging implementations are event-driven and focus on on-demand data acquisition to reduce phototoxicity, we here present ‘outcome-driven’ microscopy, a framework combining smart microscopy with optogenetics to control cell biological processes and achieve predefined outcomes. We validate this approach using light-based control of cell migration and nucleocytoplasmic transport, demonstrating robust spatiotemporal control of cellular behaviour in single cells and in cell populations.
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