Compartmentalized cross-linked enzyme nano aggregates (c-CLEnAs) toward pharmaceutical transformations

Journal article (2021)

Authors

M. Teresa de Martino Eindhoven University of Technology
F. Tonin BT/Biocatalysis - AS
Victor R.L.J. Bloemendal Radboud Universiteit Nijmegen, Eindhoven University of Technology
U. Hanefeld BT/Biocatalysis - AS
Floris P J T Rutjes Radboud Universiteit Nijmegen
Jan C.M. Van Hest Eindhoven University of Technology
Research Group
BT/Biocatalysis (AS) (TU Delft)
Copyright: © 2021 M. Teresa de Martino, F. Tonin, Victor R.L.J. Bloemendal, U. Hanefeld, Floris P.J.T. Rutjes, Jan C.M. van Hest
DOI: https://doi.org/10.1039/d1ra04332c
More Info
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Published Date

2021

Language

English

Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Biocatalysis

Abstract

A new immobilization strategy using compartmentalized nanoreactors is herein reported for two biocatalytic processes: (1)N-acetylneuraminate lyase (NAL) is internalized in NAL-c-CLEnAs and used in a continuous flow aldol condensation ofN-acetyl-d-mannosamine with sodium pyruvate toN-acetylneuraminic acid; (2) two hydroxysteroid dehydrogenases (HSDH) 7α- and 7β-HSDH are incorporated inc-CLEnAs and used in a two-step cascade batch synthesis of ursodeoxycholic acid (UDCA). The versatile use ofc-CLEnA demonstrates that this immobilization methodology is a valuable addition to the toolbox of synthetic chemists.