Compartmentalized cross-linked enzyme nano aggregates (c-CLEnAs) toward pharmaceutical transformations
M. Teresa De Martino (Eindhoven University of Technology)
F. Tonin (TU Delft - BT/Biocatalysis)
Victor R.L.J. Bloemendal (Radboud Universiteit Nijmegen, Eindhoven University of Technology)
Ulf Hanefeld (TU Delft - BT/Biocatalysis)
Floris P.J.T. Rutjes (Radboud Universiteit Nijmegen)
Jan C.M. Van Hest (Eindhoven University of Technology)
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Abstract
A new immobilization strategy using compartmentalized nanoreactors is herein reported for two biocatalytic processes: (1)N-acetylneuraminate lyase (NAL) is internalized in NAL-c-CLEnAs and used in a continuous flow aldol condensation ofN-acetyl-d-mannosamine with sodium pyruvate toN-acetylneuraminic acid; (2) two hydroxysteroid dehydrogenases (HSDH) 7α- and 7β-HSDH are incorporated inc-CLEnAs and used in a two-step cascade batch synthesis of ursodeoxycholic acid (UDCA). The versatile use ofc-CLEnA demonstrates that this immobilization methodology is a valuable addition to the toolbox of synthetic chemists.
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