PDGFRβ<sup>+</sup> cells play a dual role as hematopoietic precursors and niche cells during mouse ontogeny

Journal article (2022)

Authors

Diana Sá da Bandeira The University of Edinburgh
Alastair Morris Kilpatrick The University of Edinburgh
Madalena Marques The University of Edinburgh
Mario Gomez-Salazar The University of Edinburgh
Telma Ventura The University of Edinburgh
Zaniah Nashira Gonzalez The University of Edinburgh
Dorota Stefancova The University of Edinburgh
Fiona Rossi The University of Edinburgh
C.S. Vink The University of Edinburgh
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Pericytes AGM single-cell RNA-sequencing Bmp4 CP: Developmental biology CP: Stem cell research Hematopoietic niche HSPC precursor MSCs Osteogenesis PDGFRβ VSMCs
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Published Date

2022

Language

English

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Abstract

Hematopoietic stem cell (HSC) generation in the aorta-gonad-mesonephros region requires HSC specification signals from the surrounding microenvironment. In zebrafish, PDGF-B/PDGFRβ signaling controls hematopoietic stem/progenitor cell (HSPC) generation and is required in the HSC specification niche. Little is known about murine HSPC specification in vivo and whether PDGF-B/PDGFRβ is involved. Here, we show that PDGFRβ is expressed in distinct perivascular stromal cell layers surrounding the mid-gestation dorsal aorta, and its deletion impairs hematopoiesis. We demonstrate that PDGFRβ+ cells play a dual role in murine hematopoiesis. They act in the aortic niche to support HSPCs, and in addition, PDGFRβ+ embryonic precursors give rise to a subset of HSPCs that persist into adulthood. These findings provide crucial information for the controlled production of HSPCs in vitro.