A compact microscope for voltage imaging
X. Meng (TU Delft - ImPhys/Microscopy Instrumentation & Techniques)
Lex Huismans (Student TU Delft)
Teun A.P.M. Huijben (TU Delft - ImPhys/Computational Imaging)
Greta Szabó (TU Delft - ImPhys/Microscopy Instrumentation & Techniques)
Ruud van Tol (TU Delft - EMSD AS-south Project engineers)
Izak De Heer (Student TU Delft)
Srividya Ganapathy (TU Delft - ImPhys/Microscopy Instrumentation & Techniques)
Daan Brinks (Erasmus MC, TU Delft - ImPhys/Microscopy Instrumentation & Techniques)
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Abstract
Voltage imaging and optogenetics offer new routes to optically detect and influence neural dynamics. Optimized hardware is necessary to make the most of these new techniques. Here we present the Octoscope, a versatile, multimodal device for all-optical electrophysiology. We illustrate its concept and design and demonstrate its capability to perform both 1-photon and 2-photon voltage imaging with spatial and temporal light patterning, in both inverted and upright configurations, in vitro and in vivo.
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