Extracellular vesicle quantification and characterization
Common methods and emerging approaches
Thomas A. Hartjes (Erasmus MC)
Serhii Mytnyk (TU Delft - ChemE/Product and Process Engineering)
G. Jenster (Erasmus MC)
Volkert Van Steijn (TU Delft - ChemE/Product and Process Engineering)
Martin E. van Royen (Erasmus MC)
More Info
expand_more
Other than for strictly personal use, it is not permitted to download, forward or distribute the text or part of it, without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license such as Creative Commons.
Abstract
Extracellular vesicles (EVs) are a family of small membrane vesicles that carry information about cells by which they are secreted. Growing interest in the role of EVs in intercellular communication, but also in using their diagnostic, prognostic and therapeutic potential in (bio) medical applications, demands for accurate assessment of their biochemical and physical properties. In this review, we provide an overview of available technologies for EV analysis by describing their working principles, assessing their utility in EV research and summarising their potential and limitations. To emphasise the innovations in EV analysis, we also highlight the unique possibilities of emerging technologies with high potential for further development.