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Assessing the thiamine diphosphate dependent pyruvate dehydrogenase E1 subunit for carboligation reactions with aliphatic ketoacids

Journal Article (2020)
Author(s)

Stefan R. Marsden (TU Delft - BT/Biocatalysis)

D.G.G. McMillan (TU Delft - BT/Biocatalysis)

U. Hanefeld (TU Delft - BT/Biocatalysis)

Research Group
BT/Biocatalysis
Copyright
© 2020 S.R. Marsden, D.G.G. McMillan, U. Hanefeld
DOI related publication
https://doi.org/10.3390/ijms21228641
More Info
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Publication Year
2020
Language
English
Copyright
© 2020 S.R. Marsden, D.G.G. McMillan, U. Hanefeld
Research Group
BT/Biocatalysis
Issue number
22
Volume number
21
Pages (from-to)
1-15
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Abstract

The synthetic properties of the Thiamine diphosphate (ThDP)-dependent pyruvate dehydrogenase E1 subunit from Escherichia coli (EcPDH E1) was assessed for carboligation reactions with aliphatic ketoacids. Due to its role in metabolism, EcPDH E1 was previously characterised with respect to its biochemical properties, but it was never applied for synthetic purposes. Here, we show that EcPDH E1 is a promising biocatalyst for the production of chiral α-hydroxyketones. WT EcPDH E1 shows a 180–250-fold higher catalytic efficiency towards 2-oxobutyrate or pyruvate, respectively, in comparison to engineered transketolase variants from Geobacillus stearothermophilus (TKGST). Its broad active site cleft allows for the efficient conversion of both (R)-and (S)-configured α-hydroxyaldehydes, next to linear and branched aliphatic aldehydes as acceptor substrates under kinetically controlled conditions. The alternate, thermodynamically controlled self-reaction of aliphatic aldehydes was shown to be limited to low levels of conversion, which we propose to be due to their large hydration constants. Additionally, the thermodynamically controlled approach was demonstrated to suffer from a loss of stereoselectivity, which makes it unfeasible for aliphatic substrates.