Acid phosphatase behaviour at an electrified soft junction and its interfacial co-deposition with silica

Journal article (2018)

Authors

L. Poltorak OLD ChemE/Organic Materials and Interfaces
N. van der Meijden Applied Sciences
S. Oonk Netherlands Forensic Institute - NFI
Ernst J. R. Sudhölter OLD ChemE/Organic Materials and Interfaces
M. de Puit OLD ChemE/Organic Materials and Interfaces , Netherlands Forensic Institute - NFI
Research Group
OLD ChemE/Organic Materials and Interfaces
Copyright: © 2018 L. Poltorak, N. van der Meijden, S. Oonk, Ernst J. R. Sudhölter, M. de Puit
DOI
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https://doi.org/10.1016/j.elecom.2018.07.022
Proteins Acid phosphatase Interfacial deposition ITIES Sol-gel process of silica
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Published Date

01-09-2018

Language

English

Research Group

OLD ChemE/Organic Materials and Interfaces

Abstract

The behaviour of acid phosphatase at an electrified liquid–liquid interface was studied in this work. It was found that only the protonated form of the protein can undergo interfacial adsorption which is affected by the pH of the aqueous phase. With ion transfer voltammetry we could detect acid phosphatase in concentrations as low as 0.1 μM. We were able to co-deposit the protein and silica at the electrified liquid–liquid interface via controlled proton transfer to the organic phase where it catalyzed tetraethoxysilane hydrolysis, followed by polycondensation to silica.