Practical guidelines for implementing adaptive optics in fluorescence microscopy
D. Wilding (TU Delft - Team Raf Van de Plas)
P. Pozzi (TU Delft - Team Raf Van de Plas)
OA Soloviev (TU Delft - Team Raf Van de Plas)
Gleb Vdovine (TU Delft - Team Raf Van de Plas)
Michel Verhaegen (TU Delft - Team Raf Van de Plas)
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Abstract
In life sciences, interest in the microscopic imaging of increasingly complex three dimensional samples, such as cell spheroids, zebrafish embryos, and in vivo applications in small animals, is growing quickly. Due to the increasing complexity of samples, more and more life scientists are considering the implementation of adaptive optics in their experimental setups. While several approaches to adaptive optics in microscopy have been reported, it is often difficult and confusing for the microscopist to choose from the array of techniques and equipment. In this poster presentation we offer a small guide to adaptive optics providing general guidelines for successful adaptive optics implementation.